RKO Cell Line | CL-0196

RKO Cell Line | CL-0196 | Gentaur US, UK & Europe Disrtribition

Media: CM-0196

Organism: Homo sapiens, Human

Application: It can be used as the control cell line for investigating the effects of p53 and gadd45 on cellular parameters.

Background: RKO cells contain wild-type p53 but lack endogenous human thyroid receptor nuclear receptor(h-TRbeta1). The level of p53 protein is higher in RKO cells than in RKO-E6 cells. The RKO cell line is the parental cell line(isogenic) of RKO-E6 and RKO-A545-1.

Age: N/A

Tissue: Colon

Morphology: Epithelial

Growth Properties: Adherent

Doubling Time: N/A

Biosafty: 1

Medium: MEM(PM150410)+10% FBS+1% P/S(PB180120)

Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Ratio: 1:2 to 1:4

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: Yes, in nude mice. Yes, in soft agar.

Duration: N/A

Lead Time: 3-5 days