Description
RKO-AS45-1 cell line | CL-0425 | Gentaur US, UK & Europe Disrtribition
Media: CM-0425
Organism: Homo sapiens, human
Application: N/A
Background: The RKO-AS45-1 cell line was generated from the colon carcinoma RKO cell line by transfection with the open reading frame portions of human cDNAs for GADD45 cloned into the expression vector pCMV.
Age: N/A
Tissue: Colon
Morphology: Epithelial
Growth Properties: Adherent
Doubling Time: N/A
Biosafty: 2
Medium: MEM(PM150410)+10% FBS+1% P/S(PB180120)
Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
Ratio: 1:2 to 1:5
Renewal: Every 2 to 3 days
Cryopreservation: Freeze Medium: 55% Basal Medium+40% FBS+5% DMSO Storage Temperature: Liquid Nitrogen Vapor Phase
Culture Conditions: Atmosphere: Air, 95%; CO2, 5%; Temperature: 37℃
Effects: N/A
Duration: N/A
Lead Time: 3-5 days
