Description
Retinoblastoma Antibody / Rb1 | V3578-20UG | Gentaur US, UK & Europe Disrtribition
Family: Primary antibody
Formulation: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Format: Purified
Clone: RB1/2313R
Host: Rabbit
Clonality: Recombinant Rabbit Monoclonal
Isotype: Rabbit IgG, kappa
Species Reactivity: Human, Mouse
Application: IHC-P
Application Details: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note: Due to variation in protocol and secondary antibody used, the recombinant RB1 antibody may need to be titered for optimal performance.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Purity: Protein A affinity chromatography
Description: Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.
Immunogen: A human recombinant partial protein was used as immunogen for this recombinant RB1 antibody.
Storage: The recombinant RB1 antibody (with azide) can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20 °C or colder.
Localization: Nuclear
