Renca [RenCa] Cell Line | CL-0568

Renca [RenCa] Cell Line | CL-0568 | Gentaur US, UK & Europe Disrtribition

Media: CM-0568

Organism: Mus musculus, Mouse

Application: N/A

Background: The pattern of growth of this tumor accurately mimics that of human adult renal cell carcinoma, particularly with regard to spontaneous metastasis to lung and liver. The cells do not express transforming growth factor-beta type II receptor (TbetaR-II).

Age: 6 weeks

Tissue: Kidney

Morphology: Epithelial-like

Growth Properties: Adherent

Doubling Time: 24h

Biosafty: 1

Medium: RPMI-1640(PM150110)+10% FBS+1% P/S(PB180120)+0.1mM NEAA(PB180424)+1mM Sodium Pyruvate+2mM L-glutamine

Subculturing: Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.

Ratio: 1:2 to 1:4

Renewal: Every 2 to 3 days

Cryopreservation: Freeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase

Culture Conditions: Atmosphere: Air, 95%; CO2, 5% Temperature: 37℃

Effects: N/A

Duration: N/A

Lead Time: 3-5 days