Recombinant ZIKV (strain Zika SPH2015) Envelope protein (Domain III, His Tag) | PKSV030271

Recombinant ZIKV (strain Zika SPH2015) Envelope protein (Domain III, His Tag) | PKSV030271 | Gentaur US, UK & Europe Disrtribition

Synonyms: Zika virus, E protein, strain Zika SPH2015

Active Protein: N/A

Activity: A DNA sequence encoding the Zika virus (strain Zika SPH2015) E / Envelope (domain III) (ALU33341.1) (Val593-Lys699) was expressed with a polyhistidine tag at the C-terminus.

Protein Construction: A DNA sequence encoding the Zika virus (strain Zika SPH2015) E / Envelope (domain III) (ALU33341.1) (Val593-Lys699) was expressed with a polyhistidine tag at the C-terminus.

Fusion Tag: C-His

Species: ZIKV

Expressed Host: HEK293 Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin: Please contact us for more information.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 13kDa.

Formulation: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.

Reconstitution: Please refer to the printed manual for detailed information.

Background: Envelope of Zika virus is resposible for receptor binding and membrane. Analysis of the envelope protein of Zika, from Brazilian Zika SPH215 (KU321639), indicates predicted B and T cell epitopes in peptides that are consistent to those reported for dengue, YFYF and Japanese encephalitis. The envelope Domain II B cell epitope, to which much dengue non-neutralizing cross reaction is attributed, is also conserved also in Zika virus, consistent with prior field observations of cross reactivity with dengue and YF. Domain III of the Zika envelope protein, likely the main specific neutralizing domain, is distinct from recent Brazilian dengue isolates and a recent Peruvian YF isolate (GQ379163), 76% of possible major histocompatibility complex class (MHC) I and MHC II binding peptides and potential B cell linear epitopes are unique to Zika virus.

Research Area: Virus related Protein