Description
Recombinant S. cerevisiae TIM14 Protein | PKSQ050085 | Gentaur US, UK & Europe Disrtribition
Synonyms: Mitochondrial import inner membrane translocase subunit TIM14; Presequencetranslocated-associated motor subunit PAM18; PAM18; TIM14
Active Protein: N/A
Activity: Recombinant S. cerevisiae TIM14 is produced by our E.coli expression system and the target gene encoding Phe99-Lys168 is expressed.
Protein Construction: Recombinant S. cerevisiae TIM14 is produced by our E.coli expression system and the target gene encoding Phe99-Lys168 is expressed.
Fusion Tag: N/A
Species: S. cerevisiae
Expressed Host: E.coli
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Purity: > 95 % as determined by reducing SDS-PAGE.
Endotoxin: < 1.0 EU per μg as determined by the LAL method.
Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Molecular Mass: 7.9 kDa
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 300mM NaCl, pH8.0.
Reconstitution: Please refer to the printed manual for detailed information.
Background: Mitochondrial import inner membrane translocase subunit TIM14 (TIM14) is an essential component of the PAM complex. PAM complex is required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. In the complex, TIM14 is required to stimulate activity of mtHSP70 (SSC1). TIM14 belongs to the DnaJ family, which has been involved in Hsp40/Hsp70 chaperone systems. As a mitochondrial chaperone, TIM14 functions as part of the TIM23 complex import motor to facilitate the import of nuclear-encoded proteins into the mitochondria. TIM14 also complexes with prohibitin complexes to regulate mitochondrial morphogenesis, and has been implicated in dilated cardiomyopathy with ataxia.
Research Area: N/A
