Recombinant Rat DNMT1 Protein (His Tag) | PKSR040470

Recombinant Rat DNMT1 Protein (His Tag) | PKSR040470 | Gentaur US, UK & Europe Disrtribition

Synonyms: DNA (cytosine-5)-methyltransferase 1;Dnmt1;Met-1;DNA methyltransferase MmuI;DNA MTase MmuI;M.MmuI;MCMT;Dnmt; Met1; Uim

Active Protein: N/A

Activity: A DNA sequence encoding the Rat DNMT1 (Q9Z330-1) (Phe950-Pro1142) was expressed with a polyhistidine tag at the N-terminus.

Protein Construction: A DNA sequence encoding the Rat DNMT1 (Q9Z330-1) (Phe950-Pro1142) was expressed with a polyhistidine tag at the N-terminus.

Fusion Tag: N-His

Species: Rat

Expressed Host: E.coli

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 80 % as determined by reducing SDS-PAGE.

Endotoxin: Please contact us for more information.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 22.01 kDa

Formulation: Lyophilized from sterile PBS, pH 7.4

Reconstitution: Please refer to the printed manual for detailed information.

Background: Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells. Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing. Promotes tumor growth.

Research Area: N/A