Description
Recombinant Mouse Sonic Hedgehog/SHH (C-6His) | PKSM041416 | Gentaur US, UK & Europe Disrtribition
Synonyms: Sonic Hedgehog Protein; SHH; HHG-1; SHH
Active Protein: N/A
Activity: Recombinant Mouse Sonic Hedgehog is produced by our Mammalian expression system and the target gene encoding Cys25Gly198 is expressed with a 6His tag at the C-terminus.
Protein Construction: Recombinant Mouse Sonic Hedgehog is produced by our Mammalian expression system and the target gene encoding Cys25Gly198 is expressed with a 6His tag at the C-terminus.
Fusion Tag: C-6His
Species: Mouse
Expressed Host: Human Cells
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Purity: > 95 % as determined by reducing SDS-PAGE.
Endotoxin: < 1.0 EU per µg as determined by the LAL method.
Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Molecular Mass: 20.4 kDa
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, 5% Trehalose, pH 7.4.
Reconstitution: Please refer to the printed manual for detailed information.
Background: Mouse Sonic Hedgehog Homolog (SHH) belongs to a three-protein family called Hedgehog. The other two family members are Indian Hedgehog (IHH) and Desert Hedgehog (DHH). Hedgehog proteins are key signaling molecules in embryonic development. SHH is expressed in various embryonic tissues and plays critical roles in regulating the patterning of many systems, such as limbs and brain. SHH also plays an important role in adult, including the division of adult stem cells and the development of certain cancers and other diseases.Mouse Shh is synthesized as a 437 aa precursor that contains a 24 aa signal sequence and a 413 aa mature region. The mature region is autocatalytically processed into a nonglycosylated, 20 kDa, 174 aa Nterminal fragment (ShhN), and a catalyticprocessing, glycosylated, 34 kDa, 239 aa Cterminal fragment. The 20 kDa ShhN fragment is the core of the active hedgehog molecule. Mouse ShhN is 99%, 98%, and 100% aa identical to human, rat and gerbil ShhN, respectively.
Research Area: N/A
