Recombinant Mouse SHP2/PTPN11 Protein (His Tag)(Active) | PKSM040671

Recombinant Mouse SHP2/PTPN11 Protein (His Tag)(Active) | PKSM040671 | Gentaur US, UK & Europe Disrtribition

Synonyms: 2700084A17Rik;AW536184;PTP1D;PTP2C;SAP-2;SH-PTP2;SH-PTP3;SHP-2;Shp2;Syp

Active Protein: Active protein

Activity: A DNA sequence encoding the mouse PTPN11 (P35235-2) (Met1-Arg593) was expressed with a C-terminal polyhistidine tag.

Protein Construction: A DNA sequence encoding the mouse PTPN11 (P35235-2) (Met1-Arg593) was expressed with a C-terminal polyhistidine tag.

Fusion Tag: C-His

Species: Mouse

Expressed Host: HEK293 Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 69.5 kDa

Formulation: Lyophilized from sterile 20mM Tris, 0.1M NaCl, 10%Glycerol, 1mM TCEP, 5% trehalose, 5%mannitol, 0.01% tween-80

Reconstitution: Please refer to the printed manual for detailed information.

Background: SHP2, also known as PTPN11, belongs to the protein-tyrosine phosphatase(PTP) family, non-receptor class 2 subfamily. PTPs catalyze the removal of phosphate groups from tyrosine residues by the hydrolysis of phosphoric acid monoesters. They dephosphorylate EGFR, JAK2 and TYK2 kinases, promoting oncogenic transformation. SHP2 is widely expressed, with highest levels in heart, brain, and skeletal muscle. SHP2 acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus. It also dephosphorylates ROCK2 at Tyr-722 resulting in stimulatation of its RhoA binding activity.

Research Area: N/A