Recombinant Mouse R-Spondin 1/RSPO1 Protein (His Tag)(Active) | PKSM040742

Recombinant Mouse R-Spondin 1/RSPO1 Protein (His Tag)(Active) | PKSM040742 | Gentaur US, UK & Europe Disrtribition

Synonyms: R-spondin;Rspondin

Active Protein: Active protein

Activity: A DNA sequence encoding the full length of mouse RSPO1 (NP_619624.2) (Met 1-Gln 265) was fused with a polyhistidine tag at the C-terminus.

Protein Construction: A DNA sequence encoding the full length of mouse RSPO1 (NP_619624.2) (Met 1-Gln 265) was fused with a polyhistidine tag at the C-terminus.

Fusion Tag: C-His

Species: Mouse

Expressed Host: CHO Stable Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 28.5 kDa

Formulation: Lyophilized from sterile PBS, pH 7.4

Reconstitution: Please refer to the printed manual for detailed information.

Background: RSPO1 gene is a member of the R-spondin family. It encodes RSPO1 which is known as a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, RSPO1 induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. This protein is an activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. RSPO1 acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. It also acts as a ligand for frizzled FZD8 and LRP6.

Research Area: N/A