Recombinant Mouse PLA2G12B/PLA2G13 Protein (His Tag) | PKSM040547

Recombinant Mouse PLA2G12B/PLA2G13 Protein (His Tag) | PKSM040547 | Gentaur US, UK & Europe Disrtribition

Synonyms: 2010002E04Rik;Fksg71;hlb218;Pla2g13

Active Protein: N/A

Activity: A DNA sequence encoding the mouse PLA2G12B (NP_076019.2) (Met 1-Leu 195) was expressed, with a C-terminal polyhistidine tag.

Protein Construction: A DNA sequence encoding the mouse PLA2G12B (NP_076019.2) (Met 1-Leu 195) was expressed, with a C-terminal polyhistidine tag.

Fusion Tag: C-His

Species: Mouse

Expressed Host: HEK293 Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 21 kDa

Formulation: Lyophilized from sterile 20mM NaAc, 100mM NaCl, pH 5.0

Reconstitution: Please refer to the printed manual for detailed information.

Background: Group XIIB secretory phospholipase A2-like protein, also known as Group XIII secretory phospholipase A2-like protein, GXIII sPLA2-like, sPLA2-GXIIB, GXIIB, PLA2G13 and PLA2G12B, is a secreted protein which belongs to the phospholipase A2 family. PLA2G12B / PLA2G13 is strong expression in liver, small intestine and kidney. Mammalian secretory phospholipase A2s ( sPLA2s ) form a family of structurally related enzymes that are involved in a variety of physiological and pathological processes via the release of arachidonic acid from membrane phospholipids or the binding to specific membrane receptors. Phospholipases A2 / PLA2 are enzymes that release fatty acids from the second carbon group of glycerol. This particular phospholipase specifically recognizes the sn-2 acyl bond of phospholipids and catalytically hydrolyzes the bond releasing arachidonic acid and lysophospholipids. Phospholipases A2 / PLA2 are commonly found in mammalian tissues as well as insect and snake venom. Venom from both snakes and insects is largely composed of melittin, which is a stimulant of Phospholipases A2 / PLA2. Due to the increased presence and activity of Phospholipases A2 / PLA2 resulting from a snake or insect bite, arachidonic acid is released from the phospholipid membrane disproportionately. As a result, inflammation and pain occur at the site.

Research Area: N/A