Recombinant Mouse PARP-1 Protein (His Tag)(Active) | PKSM040501

Recombinant Mouse PARP-1 Protein (His Tag)(Active) | PKSM040501 | Gentaur US, UK & Europe Disrtribition

Synonyms: 5830444G22Rik;Adprp;Adprt1;AI893648;ARTD1;C80510;PARP;parp-1;PPOL;sPARP-1

Active Protein: Active protein

Activity: A DNA sequence encoding the mouse PARP1 (NP_031441.2) (Met 1-Trp 1014) was fused with a polyhistidine tag at the N-terminus.

Protein Construction: A DNA sequence encoding the mouse PARP1 (NP_031441.2) (Met 1-Trp 1014) was fused with a polyhistidine tag at the N-terminus.

Fusion Tag: N-His

Species: Mouse

Expressed Host: Baculovirus-Insect Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 85 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 115 kDa

Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly, 0.1mM TCEP

Reconstitution: Please refer to the printed manual for detailed information.

Background: Poly (ADP-ribose) polymerase 1(PRAP1), also known as NAD(+) ADP-ribosyltransferase 1(ADPRT), is a chromatin-associated enzyme which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The ADP-D-ribosyl group of NAD+ is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. The poly(ADP-ribosyl)ation modification is critical for a wide range of processes, including DNA repair, regulation of chromosome structure, transcriptional regulation, mitosis and apoptosis. PARP1 is demonstrateed to mediate the poly(ADP-ribose) ation of APLF (aprataxin PNK-like factor) and CHFR (checkpoint protein with FHA and RING domains), two representative proteins involved in the DNA damage response and checkpoint regulation. Further, It has been suggested that DNA-dependent protein kinase (DNA-PK), another component of DNA repair, suppresses PARP activity, probably through direct binding and/or sequestration of DNA-ends which serve as an important stimulator for both enzymes. PARP1 inhibitors is thus proposed as a targeted cancer therapy for recombination deficient cancers, such as BRCA2 tumors.Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Research Area: N/A