Recombinant Mouse Motch A/NOTCH1 Protein (His Tag)(Active) | PKSM040558

Recombinant Mouse Motch A/NOTCH1 Protein (His Tag)(Active) | PKSM040558 | Gentaur US, UK & Europe Disrtribition

Synonyms: Neurogenic locus notch homolog protein 1; Notch 1; Motch A; Mt14;lin-12;Mis6;N1;Tan1

Active Protein: Active protein

Activity: A DNA sequence encoding the mouse NOTCH1 (NP_032740.3) N-terminal fragment (Met 1-Gln 526) was expressed，with a C-terminal polyhistidine tag.

Protein Construction: A DNA sequence encoding the mouse NOTCH1 (NP_032740.3) N-terminal fragment (Met 1-Gln 526) was expressed，with a C-terminal polyhistidine tag.

Fusion Tag: C-His

Species: Mouse

Expressed Host: Baculovirus-Insect Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 82 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 55 kDa

Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly

Reconstitution: Please refer to the printed manual for detailed information.

Background: NOTCH1 is one of the four mammalian Notch receptors, which is involved in the Notch signaling pathway. Specifically, NOTCH1 promotes the proliferation of myogenic precursor cells, and the NICD domain of NOTCH1 can impair regeneration of skeletal muscles.NOTCH1 is a prevalent signaling pathway in T cell acute lymphoblastic leukemia (T-ALL).The NOTCH signaling pathway is a conserved signaling cascade that regulates many aspects of development and homeostasis in multiple organ systems.The proto-oncogene NOTCH1 is frequently mutated in around 1% of patients with chronic lymphocytic leukemia (CLL). NOTCH1 mutations in oral squamous cell carcinoma (OSCC) frequently occur near the ligand-binding region. These mutations change the domain structure of this protein and affect the ligand binding activity. When NOTCH1 is activated by ligand binding, NOTCH1 intracellular domain (NICD) is cleaved from the cell membrane.

Research Area: N/A