Recombinant Mouse METAP2 Protein (His Tag)(Active) | PKSM040634

Recombinant Mouse METAP2 Protein (His Tag)(Active) | PKSM040634 | Gentaur US, UK & Europe Disrtribition

Synonyms: 4930584B20Rik;A930035J23Rik;AI047573;AL024412;Amp2;AU014659;Mnpep;p67;p67eIF2

Active Protein: Active protein

Activity: A DNA sequence encoding the mouse METAP2 (O08663) (Ala 2-Tyr 478) was expressed，with a C-terminal polyhistidine tag.

Protein Construction: A DNA sequence encoding the mouse METAP2 (O08663) (Ala 2-Tyr 478) was expressed，with a C-terminal polyhistidine tag.

Fusion Tag: C-His

Species: Mouse

Expressed Host: Baculovirus-Insect Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 88 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 54.3 kDa

Formulation: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 10% glycerol

Reconstitution: Please refer to the printed manual for detailed information.

Background: METAP2 (Methionine aminopeptidase 2), also known as MAP2 is a a protein which belongs to the peptidase M24A family. MAP2 binds 2 cobalt or manganese ions and contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. It is found in all organisms and is especially important because of its critical role in tissue repair and protein degradation. The catalytic activity of human MAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. MAP2 protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. It also plays a critical role in the regulation of protein synthesis.

Research Area: N/A