Recombinant Mouse Legumain/LGMN Protein (His Tag) | PKSM041191

Recombinant Mouse Legumain/LGMN Protein (His Tag) | PKSM041191 | Gentaur US, UK & Europe Disrtribition

Synonyms: Legumain;Lgmn;Asparaginyl endopeptidase;Protease cysteine 1;Prsc1;AEP

Active Protein: N/A

Activity: Recombinant Mouse Legumain/Asparaginyl Endopeptidase is produced by our Mammalian expression system and the target gene encoding Val18-Tyr435 is expressed with a 6His tag at the C-terminus.

Protein Construction: Recombinant Mouse Legumain/Asparaginyl Endopeptidase is produced by our Mammalian expression system and the target gene encoding Val18-Tyr435 is expressed with a 6His tag at the C-terminus.

Fusion Tag: C-6His

Species: Mouse

Expressed Host: Human Cells

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per μg as determined by the LAL method.

Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Molecular Mass: 48.7 kDa

Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, 20% Glycerol, pH8.0.

Reconstitution: Not Applicable

Background: Mouse Legumain, also known as LGMN, is a cysteine protease belonging to peptidase family C13 and is expressed in kidney and placenta abundantly. LGMN has a strict specificity for hydrolysis of asparaginyl bonds. It can also cleave aspartyl bonds slowly, especially under acidic conditions. The mammalian legumain is involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. It plays a role in the regulation of cell proliferation via its role in EGFR degradation. Legumain deficiency causes the accumulation of pro-Cathepsins B, H and L, another group of lysosomal cysteine proteases. Overexpression of Legumain in tumors is significant for invasion/metastasis. Mammalian legumain is inhibited by iodoacetamide and maleimides. Legumain activation appears to be autocatalytic and can be triggered by acidic pH.

Research Area: N/A