Recombinant Mouse ENPP7/NPP-7 Protein (His Tag) | PKSM040611

Recombinant Mouse ENPP7/NPP-7 Protein (His Tag) | PKSM040611 | Gentaur US, UK & Europe Disrtribition

Synonyms: Alk-SMase;Gm254

Active Protein: N/A

Activity: A DNA sequence encoding the mouse ENPP7 (Q3TIW9) (Met 1-Gln 421) was expressed, with a polyhistidine tag at the C-terminus.

Protein Construction: A DNA sequence encoding the mouse ENPP7 (Q3TIW9) (Met 1-Gln 421) was expressed, with a polyhistidine tag at the C-terminus.

Fusion Tag: C-His

Species: Mouse

Expressed Host: HEK293 Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 47 kDa

Formulation: Lyophilized from sterile 20mM Tris, 150mM NaCl, pH 7.6

Reconstitution: Please refer to the printed manual for detailed information.

Background: Ectonucleotide pyrophosphatase / phosphodiesterase family member 7, also known as Alkaline sphingomyelin phosphodiesterase, Intestinal alkaline sphingomyelinase, Alk-Smase, ENPP7 and NPP-7, is a single-pass type I membrane protein which belongs to the nucleotide pyrophosphatase / phosphodiesterase family. ENPP7 / NPP-7 is expressed in the intestines and human bile. ENPP7 / NPP-7 is localized at the surface of the microvillar membrane in small intestine enterocytes, as well as in endosome-like structures and in Golgi complex. The main function of ENPP7 / NPP-7 is to convert the dietary sphingomyelin into ceramide, the sphingolipid messengers via hydrolyzation. ENPP7 / NPP-7 is also reported to exert a phospholipase C activity toward palmitoyl lyso-phosphocholine. The activity of this enzyme is inhibited in a dose dependent manner by ATP, imidazole, orthovanadate and zinc ion. Further, It has been shown in studies that decreased levels of ENPP7 / NPP-7 may be associated with human colon cancer.

Research Area: N/A