Recombinant Mouse DOPA Decarboxylase/DDC Protein (His Tag) | PKSM040483

Recombinant Mouse DOPA Decarboxylase/DDC Protein (His Tag) | PKSM040483 | Gentaur US, UK & Europe Disrtribition

Synonyms: Aadc

Active Protein: N/A

Activity: A DNA sequence encoding the mouse DDC (O88533) (Met 1-Glu 480) was expressed，with a C-terminal polyhistidine tag.

Protein Construction: A DNA sequence encoding the mouse DDC (O88533) (Met 1-Glu 480) was expressed，with a C-terminal polyhistidine tag.

Fusion Tag: C-His

Species: Mouse

Expressed Host: Baculovirus-Insect Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 55.2 kDa

Formulation: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 20% gly, 3mM DTT

Reconstitution: Please refer to the printed manual for detailed information.

Background: Dopa Decarboxylase (DDC), also known as AADC and Aromatic-L-amino acid decarboxylase, is a 54 kDa member of the group II decarboxylase family of proteins.It is a vitamin B6-dependent homodimeric enzyme that catalyzes the decarboxylation of both L-3, 4-dihydroxyphenylalanine (L-DOPA) and L-5-hydroxytryptophan to dopamine and serotonin, respectively, which are major mammalian neurotransmitters and hormones belonging to catecholamines and indoleamines. Since L-DOPA is regularly used to treat the symptoms of Parkinson's disease, the catalytic pathway is of particular research interest. Defects of DDC are associated with severe developmental delay, oculogyric crises (OGC), as well as autosomal recessive disorder AADC deficiency, an early onset inborn error in neurotransmitter metabolism which can lead to catecholamine and serotonin deficiency.

Research Area: N/A