Recombinant Mouse DDR1 Kinase/MCK10 Protein (His &GST Tag)(Active) | PKSM040294

Recombinant Mouse DDR1 Kinase/MCK10 Protein (His &GST Tag)(Active) | PKSM040294 | Gentaur US, UK & Europe Disrtribition

Synonyms: 6030432F18;AI323681;Cak;CD167a;Nep;PTK3A

Active Protein: Active protein

Activity: A DNA sequence encoding the mouse DDR1 (Q03146-2) (Leu444-Val874) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Protein Construction: A DNA sequence encoding the mouse DDR1 (Q03146-2) (Leu444-Val874) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Fusion Tag: N-His-GST

Species: Mouse

Expressed Host: Baculovirus-Insect Cells

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Molecular Mass: 75.8kDa

Formulation: Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol, 2mM DTT

Reconstitution: Not Applicable

Background: Discoidin domain receptor family, member 1 (DDR1), also known as or CD167a (cluster of differentiation 167a), and Mammary carcinoma kinase 10 (MCK10), belongs to a subfamily of tyrosine kinase receptors with an extracellular domain homologous to Dictyostellium discoideum protein discoidin 1. Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism. Expression of DDR1/MCK10/CD167 is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. DDR1/MCK10/CD167 plays an important role in regulating attachment to collagen, chemotaxis, proliferation, and MMP production in smooth muscle cells. DDR1 functions in a feedforward loop to increase p53 levels and at least some of its effectors. Inhibition of DDR1 function resulted in strikingly increased apoptosis of wild-type p53-containing cells in response to genotoxic stress through a caspase-dependent pathway.

Research Area: N/A