Recombinant Mouse CXCL12/SDF-1 Protein | PKSM040993

Recombinant Mouse CXCL12/SDF-1 Protein | PKSM040993 | Gentaur US, UK & Europe Disrtribition

Synonyms: Cxcl12;Stromal cell-derived factor 1;SDF-1;12-O-tetradecanoylphorbol 13-acetate repressed protein 1;TPAR1;C-X-C motif chemokine 12;Pre-B cell growth-stimulating factor;PBSF;Thymic lymphoma cell-stimulating factor;TLSF;Sdf1

Active Protein: Active protein

Activity: Recombinant Mouse C-X-C motif chemokine 12 is produced by our E.coli expression system and the target gene encoding Lys22-Lys89 is expressed.

Protein Construction: Recombinant Mouse C-X-C motif chemokine 12 is produced by our E.coli expression system and the target gene encoding Lys22-Lys89 is expressed.

Fusion Tag: N/A

Species: Mouse

Expressed Host: E.coli

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per μg as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 8.1 kDa

Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Reconstitution: Please refer to the printed manual for detailed information.

Background: Mouse Cxcl12 is a secreted and highly conserved protein which belongs to the intercrine alpha (chemokine CxC) family.CXCL12 is widely expressed in various organs including brain, kidney, skeletal muscle, heart, liver, and lymphoid organs. Cxcl12 activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. It also binds to atypical chemokine receptor ACKR3 which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. Cxcl12 has several critical functions during embryonic development such as B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. Cxcl12 plays an important role in acting as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. It stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. It also plays a protective role after myocardial infarction, induces down-regulation and internalization of ACKR3 expressed in various cells and stimulates the proliferation of bone marrow-derived b progenitor cells in the presence of IL-7 as well as growth of the stromal cell-dependent B-cell clone DW34 cells.

Research Area: N/A