Recombinant Mouse Carbonic Anhydrase 4/CA4 Protein (His Tag)(Active) | PKSM040721

Recombinant Mouse Carbonic Anhydrase 4/CA4 Protein (His Tag)(Active) | PKSM040721 | Gentaur US, UK & Europe Disrtribition

Synonyms: CA4;CAIV;CA-IV;Car4;Carbonate dehydratase IV;carbonic anhydrase 4;carbonic anhydrase IVRP17;carbonic dehydratase IV;EC4.2.1.1;retinitis pigmentosa 17;RP17

Active Protein: Active protein

Activity: A DNA sequence encoding the mouse Car4 (NP_031633.1) without the C-terminal propeptide (Met 1-Ser 277) was expressed, with a C-terminal polyhistidine tag.

Protein Construction: A DNA sequence encoding the mouse Car4 (NP_031633.1) without the C-terminal propeptide (Met 1-Ser 277) was expressed, with a C-terminal polyhistidine tag.

Fusion Tag: C-His

Species: Mouse

Expressed Host: HEK293 Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 98 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 31 kDa

Formulation: Lyophilized from sterile PBS, pH 7.4

Reconstitution: Please refer to the printed manual for detailed information.

Background: The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase IV (CAIV) is a membrane-associated enzyme anchored to plasma membrane surfaces by a phosphatidylinositol glycan linkage. CAIV is a high-activity isozyme in CO2 hydration comparable to that of CAII. Furthermore, CAIV is more active in HCO3- dehydration than is CAII. However, the esterase activity of CAIV is decreased 150-fold compared to CAII.

Research Area: N/A