Recombinant Mouse BLMH/BLM Hydrolase Protein (His Tag)(Active) | PKSM040554

Recombinant Mouse BLMH/BLM Hydrolase Protein (His Tag)(Active) | PKSM040554 | Gentaur US, UK & Europe Disrtribition

Synonyms: AI035728;Bh;Bmh

Active Protein: Active protein

Activity: A DNA sequence encoding the mouse BLMH (NP_848760.1) (Asn 2-Glu 455) was expressed, with a polyhistide tag at the N-terminus.

Protein Construction: A DNA sequence encoding the mouse BLMH (NP_848760.1) (Asn 2-Glu 455) was expressed, with a polyhistide tag at the N-terminus.

Fusion Tag: N-His

Species: Mouse

Expressed Host: E.coli

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: Please contact us for more information.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 53.3 kDa

Formulation: Lyophilized from sterile 50mM Tris, 0.15M NaCl, 10% glycerol, pH 8.0

Reconstitution: Please refer to the printed manual for detailed information.

Background: The papain superfamily member bleomycin hydrolase (BLMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution. The only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The papain superfamily member bleomycin hydrolase (BLMH) is a neutral cysteine protease with structural similarity to a 20S proteasome. Bleomycin (BLM), a clinically used glycopeptide anticancer agent. BLMH is an essential protectant against BLM-induced death and has an important role in neonatal survival and in maintaining epidermal integrity. Sequencing revealed several putative sites phosphorylated by different types of protein kinases, but no signal sequence, transmembrane domain, N-linked glycosylation site or DNA-binding motif.

Research Area: N/A