Recombinant Human WTAP Protein (GST Tag) | PKSH030933

Recombinant Human WTAP Protein (GST Tag) | PKSH030933 | Gentaur US, UK & Europe Disrtribition

Synonyms: Mum2

Active Protein: N/A

Activity: A DNA sequence encoding the human WTAP isoform 2 (Q15007-2) (Met 1-Arg 151) was fused with the GST tag at the N-terminus.

Protein Construction: A DNA sequence encoding the human WTAP isoform 2 (Q15007-2) (Met 1-Arg 151) was fused with the GST tag at the N-terminus.

Fusion Tag: N-GST

Species: Human

Expressed Host: E.coli

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin: Please contact us for more information.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 45 kDa

Formulation: Lyophilized from sterile 20mM Tris, 0.15M NaCl, 0.5mM GSH, pH 8.0

Reconstitution: Please refer to the printed manual for detailed information.

Background: Wilms' tumor 1-associating protein (WTAP) was previously identified as a protein associated with Wilms' tumor-1 (WT-1) protein that is essential for the development of the genitourinary system. WT1 was originally identified as a tumor suppressor for Wilms' tumor, but it is also overexpressed in a variety of cancer cells. The WTAP-WT1 axis in vascular cells suggest that WTAP is a vital and multifaceted regulator of vascular remodeling. WTAP has been suggested to function in alternative splicing, stabilization of mRNA, and cell growth. Knocking down endogenous WTAP increased Smooth muscle cells (SMCs) proliferation, because of increased DNA synthesis and G(1)/S phase transition, together with reduced apoptosis. These effects could be the result of WTAP suppressing the transcriptional activity of WT1 in SMCs. WTAP may thus also play a role in messenger RNA processing in mammalian cells, either dependent on or independent of its interaction with WT1.

Research Area: N/A