Description
Recombinant Human VRK1 Protein | PKSH031090 | Gentaur US, UK & Europe Disrtribition
Synonyms: PCH1;PCH1A
Active Protein: N/A
Activity: A DNA sequence encoding the human VRK1 (Q99986) (Met 1-Lys 396) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.
Protein Construction: A DNA sequence encoding the human VRK1 (Q99986) (Met 1-Lys 396) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.
Fusion Tag:
Species: Human
Expressed Host: Baculovirus-Insect Cells
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Purity: > 95 % as determined by reducing SDS-PAGE.
Endotoxin: < 1.0 EU per µg as determined by the LAL method.
Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Molecular Mass: 45.6 kDa
Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4
Reconstitution: Please refer to the printed manual for detailed information.
Background: VRK1 is a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. Serine/threonine protein kinases are tumor suppressor that controls the activity of AMP-activated protein kinase family members; thereby playing a role in various processes such as cell metabolism; cell polarity; apoptosis and DNA damage response. VRK1 contains 1 protein kinase domain and localizes to the nucleus. VRK1 gene is widely expressed in human tissues and has increased expression in actively dividing cells; such as those in testis; thymus; fetal liver; and carcinomas. As a serine/threonine kinase; VRK1 phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Defects in VRK1 are the cause of pontocerebellar hypoplasia type 1 (PCH1); also called pontocerebellar hypoplasia with infantile spinal muscular atrophy or pontocerebellar hypoplasia with anterior horn cell disease. PCH1 is characterized by an abnormally small cerebellum and brainstem; central and peripheral motor dysfunction from birth; gliosis and anterior horn cell degeneration resembling infantile spinal muscular atrophy.
Research Area: Signal Transduction, Cell biology, epigenetics and nuclear signal,
