Recombinant Human VDR/NR1I1 Protein (His Tag) | PKSH030931

Recombinant Human VDR/NR1I1 Protein (His Tag) | PKSH030931 | Gentaur US, UK & Europe Disrtribition

Synonyms: NR1I1;PPP1R163

Active Protein: N/A

Activity: A DNA sequence encoding the human VDR (P11473) (Met 1-Ser 427) was fused with a polyhistidine tag at the C-terminus.

Protein Construction: A DNA sequence encoding the human VDR (P11473) (Met 1-Ser 427) was fused with a polyhistidine tag at the C-terminus.

Fusion Tag: C-His

Species: Human

Expressed Host: Baculovirus-Insect Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 88 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 50 KDa

Formulation: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 10% glycerol

Reconstitution: Please refer to the printed manual for detailed information.

Background: VDR (vitamin D(1, 25- dihydroxyvitamin D3)receptor), also known as NR1I1, belongs to the NR1I family, NR1 subfamily. It is composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. Vitamin D receptors (VDRs) are members of the NR1I family, which also includes pregnane X (PXR) and constitutive androstane (CAR) receptors, that form heterodimers with members of the retinoid X receptor family. VDRs repress expression of 1alpha-hydroxylase (the proximal activator of 1, 25(OH)2D3) and induce expression of the 1, 25(OH)2D3 inactivating enzyme CYP24. Also, it has recently been identified as an additional bile acid receptor alongside FXR and may function to protect gut against the toxic and carcinogenic effects of these endobiotics. VDR is expressed in the intestine, thyroid and kidney and has a vital role in calcium homeostasis. It is the nuclear hormone receptor, also called transcription factor that mediates the action of vitamin D3. Inherited mutations in the VDR gene leads to rickets.

Research Area: Signal Transduction, Cancer, epigenetics and nuclear signal,