Recombinant Human VCL/Vinculin Protein | PKSH033212

Recombinant Human VCL/Vinculin Protein | PKSH033212 | Gentaur US, UK & Europe Disrtribition

Synonyms: Vinculin; Metavinculin; VCL;CMD1W;CMH15;HEL114;MV;MVCL

Active Protein: N/A

Activity: Recombinant Human Vinculin is produced by our E.coli expression system and the target gene encoding Pro2-Gln1066 is expressed.

Protein Construction: Recombinant Human Vinculin is produced by our E.coli expression system and the target gene encoding Pro2-Gln1066 is expressed.

Fusion Tag:

Species: Human

Expressed Host: E.coli

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 117.0 kDa

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.

Reconstitution: Please refer to the printed manual for detailed information.

Background: Vinculin is a focal adhesion and cytoskeletal protein that distributed mainly at cell-cell junctions and cell-extracellular matrix (ECM) adhesion that belongs to the Vinculin/α-Catenin family. Vinculin is an Actin-binding protein and component of the Actin-Linking Functional module that senses and feels the mechanical properties of the extracellular environment. Vinculin is also a key factor that couples, transmits, transduces, and regulates mechanical force between the cytoskeleton and adhesion receptors. Vinculin generally forms two structural states, an open (active) and closed (inactive) state, which are controlled by conformational interaction(s) between the head and tail domains. Vinculin is involved in the mechano-chemical signal transmission of cells by binding to a variety of focal adhesion or cytoskeletal proteins, and plays important roles in cell adhesion, extension, motion, proliferation and survival.

Research Area: Signal Transduction, Cardiovascular, Isotypeloading control,