Description
Recombinant Human VCAM1 Protein (His Tag) | PKSH033448 | Gentaur US, UK & Europe Disrtribition
Synonyms: Vascular Cell Adhesion Protein 1; V-CAM 1; VCAM-1; INCAM-100; CD106; VCAM1; L1CAM
Active Protein: N/A
Activity: Recombinant Human Vascular Cell Adhesion Protein 1 is produced by our Mammalian expression system and the target gene encoding Phe25-Glu698 is expressed with a 6His tag at the C-terminus.
Protein Construction: Recombinant Human Vascular Cell Adhesion Protein 1 is produced by our Mammalian expression system and the target gene encoding Phe25-Glu698 is expressed with a 6His tag at the C-terminus.
Fusion Tag: C-6His
Species: Human
Expressed Host: Human Cells
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Purity: > 95 % as determined by reducing SDS-PAGE.
Endotoxin: < 1.0 EU per µg as determined by the LAL method.
Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Molecular Mass: 75.3 kDa
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 2mM CaCl2, 2mM MgCl2, 5% Threhalose, pH 7.2.
Reconstitution: Please refer to the printed manual for detailed information.
Background: VCAM-1 is a single-pass type I membrane protein, contains 7 Ig-like C2-type domains. It is an endothelial ligand for very late antigen-4 (VLA-4) and α4?7 integrin expressed on leukocytes, and thus mediates leukocyte-endothelial cell adhesion and signal transduction. VCAM-1 expression is induced on endothelial cells during inflammatory bowel disease, atherosclerosis, allograft rejection, infection, and asthmatic responses. During these responses, VCAM-1 forms a scaffold for leukocyte migration. VCAM-1 also activates signals within endothelial cells resulting in the opening of an "endothelial cell gate" through which leukocytes migrate. VCAM-1 has been identified as a potential anti-inflammatory therapeutic target, the hypothesis being that reduced expression of VCAM-1 will slow the development of atherosclerosis. In addition, VCAM-1-activated signals in endothelial cells are regulated by cytokines indicating that it is important to consider both endothelial cell adhesion molecule expression and function during inflammatory processes.
Research Area: Signal Transduction, Cardiovascular, Neuroscience, Cancer, Stem cells
