Description
Recombinant Human VAP-1/AOC3 Protein (Fc Tag) | PKSH033590 | Gentaur US, UK & Europe Disrtribition
Synonyms: Membrane primary amine oxidase; Copper amine oxidase; Semicarbazide-sensitive amine oxidase; Vascular adhesion protein 1; AOC3; VAP-1; SSAO; HPAO;VAP1
Active Protein: N/A
Activity: Recombinant Human Membrane primary amine oxidase is produced by our Mammalian expression system and the target gene encoding Arg28-Asn763 is expressed with a Fc tag at the C-terminus.
Protein Construction: Recombinant Human Membrane primary amine oxidase is produced by our Mammalian expression system and the target gene encoding Arg28-Asn763 is expressed with a Fc tag at the C-terminus.
Fusion Tag: C-Fc
Species: Human
Expressed Host: Human Cells
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Purity: > 95 % as determined by reducing SDS-PAGE.
Endotoxin: < 1.0 EU per μg as determined by the LAL method.
Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Molecular Mass: 108.5 kDa
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 500mM NaCl, pH8.0.
Reconstitution: Please refer to the printed manual for detailed information.
Background: Membrane primary amine oxidase(AOC3); also known as vascular adhesion protein (VAP-1) and HPAO; this protein is a member of the semicarbazide-sensitive amine oxidase (SSAO) family. VAP-1 is a type 1 membrane-bound glycoprotein that has a distal adhesion domain and an enzymatically active amine oxidase site outside of the membrane; VAP-1 has adhesive properties; functional monoamine oxidase activity; and possibly plays a role in glucose handling; leukocyte trafficking; and migration during inflammation. This rise in metabolic products contributes to generating advanced glycation end-products and oxidative stress along with the monoamine detoxification in the organism. It is highly expressed on the endothelium of the lung and trachea; and absent from leukocytes and epithelial cells. Membrane-bound VAP-1 releases an active; soluble form of the protein; which may be conducive to increased inflammation and the progression of many vascular disorders. In particular; elevation of VAP-1 activity and the increased enzymatic-mediated deamination is proposed to play a role in renal and vascular disease; oxidative stress; acute and chronic hyperglycemia; and diabetes complications.
Research Area: Signal Transduction, Neuroscience,
