Description
Recombinant Human UBE2M/UBC12 Protein | PKSH033478 | Gentaur US, UK & Europe Disrtribition
Synonyms: NEDD8-conjugating enzyme Ubc12; NEDD8 carrier protein; NEDD8 protein ligase; Ubiquitin-conjugating enzyme E2 M; UBC12; UBE2M;
Active Protein: N/A
Activity: Recombinant Human UBE2M is produced by our E.coli expression system and the target gene encoding Met1-Lys183 is expressed.
Protein Construction: Recombinant Human UBE2M is produced by our E.coli expression system and the target gene encoding Met1-Lys183 is expressed.
Fusion Tag: N/A
Species: Human
Expressed Host: E.coli
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.
Purity: > 95 % as determined by reducing SDS-PAGE.
Endotoxin: < 1.0 EU per μg as determined by the LAL method.
Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Molecular Mass: 20.9 kDa
Formulation: Supplied as a 0.2 μm filtered solution of 50mM HEPES, pH 7.5, 2mM DTT, 150mM NaCl, 10% Glycerol.
Reconstitution: Not Applicable
Background: UBE2M is a member of the E2 ubiquitin-conjugating enzyme family. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes; or E1s; ubiquitin-conjugating enzymes; or E2s; and ubiquitin-protein ligases; or E3s. This protein is linked with a ubiquitin-like protein; NEDD8; which can be conjugated to cellular proteins; such as Cdc53/culin. UBE2M accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1; but not RBX2; suggests that the RBX1-UBE2M complex neddylates specific target proteins; such as CUL1; CUL2; CUL3 and CUL4. It involved in cell proliferation and is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation.
Research Area: Cell biology, epigenetics and nuclear signal
