Description
Recombinant Human Troponin C/TNNC1 Protein (His Tag) | PKSH033520 | Gentaur US, UK & Europe Disrtribition
Synonyms: CMD1Z;CMH13;TN-C;TNC;TNNC
Active Protein: N/A
Activity: Recombinant Human Troponin C is produced by our E.coli expression system and the target gene encoding Met1-Glu161 is expressed with a 6His tag at the N-terminus.
Protein Construction: Recombinant Human Troponin C is produced by our E.coli expression system and the target gene encoding Met1-Glu161 is expressed with a 6His tag at the N-terminus.
Fusion Tag: N-His
Species: Human
Expressed Host: E.coli
Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.
Purity: > 95 % as determined by reducing SDS-PAGE.
Endotoxin: < 1.0 EU per μg as determined by the LAL method.
Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Molecular Mass: 19.8 kDa
Formulation: Supplied as a 0.2 μm filtered solution of 20mM Tris, 100mM NaCl, 1mM DTT, 10% glycerol, pH8.0.
Reconstitution: Not Applicable
Background: Troponin I, also known as TNI, is a 24 kDa component of a protein complex on striated muscle thin filaments.Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components: Tn-I which is the inhibitor of actomyosin ATPase, Tn-T which contains the binding site for tropomyosin and Tn-C. The binding of calcium to Tn-C abolishes the inhibitory action of Tn on actin filaments. Troponin I inhibits the calcium-dependent muscle contraction mediated by Troponins C and T. The expression of cardiac Troponin I (TNNI3) is restricted to cardiac muscle, while TNNI1 and TNNI2(encoded by distinct genes) are expressed in skeletal muscle. Mutations of cardiac Troponin I are associated with heriditary cardiomyopathy. Human cardiac Troponin I shares 93% amino acid sequence identity with mouse and rat cardiac Troponin I.
Research Area: Signal Transduction, Cardiovascular,
