Recombinant Human TK1 Protein (His Tag) | PKSH033114

Recombinant Human TK1 Protein (His Tag) | PKSH033114 | Gentaur US, UK & Europe Disrtribition

Synonyms: Thymidine kinase; cytosolic;TK1

Active Protein: N/A

Activity: Recombinant Human Thymidine kinase, Cytosolic is produced by our Mammalian expression system and the target gene encoding Met1-Asn234 is expressed with a 6His tag at the C-terminus.

Protein Construction: Recombinant Human Thymidine kinase, Cytosolic is produced by our Mammalian expression system and the target gene encoding Met1-Asn234 is expressed with a 6His tag at the C-terminus.

Fusion Tag: C-6His

Species: Human

Expressed Host: Human Cells

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Molecular Mass: 26.5 kDa

Formulation: Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 150mM NaCl, 1mM DTT, 2mM EDTA, 10% Glycerol, pH 7.5.

Reconstitution: Not Applicable

Background: Thymidine kinase 1(TK1) belongs to the thymidine kinase family. It is located in the cytoplasm, and phosphorylated on Ser-13 in mitosis during post-translational modification. Two forms of this protein have been identified in animal cells, one in cytosol TK1 and one in mitochondria TK2. Thymidine kinases have a key function in the synthesis of DNA and thereby in cell division, as they are part of the unique reaction chain to introduce deoxythymidine into the DNA. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle, while it is very low in resting cells. TK1 acts as a homotetramer, and can transform thymidime to thymidine 5'-phosphate with the help of ATP

Research Area: epigenetics and nuclear signal