Recombinant Human STAT1 Protein (His &GST Tag) | PKSH030783

Recombinant Human STAT1 Protein (His &GST Tag) | PKSH030783 | Gentaur US, UK & Europe Disrtribition

Synonyms: Signal Transducer and Activator of Transcription 1-Alpha/Beta; Transcription Factor ISGF-3 Components p91/p84; STAT1; CANDF7;IMD31A;IMD31B;IMD31C;ISGF-3;STAT91

Active Protein: N/A

Activity: A DNA sequence encoding the human STAT1 isoform beta (P42224-2) (Met 1-Val 712) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Protein Construction: A DNA sequence encoding the human STAT1 isoform beta (P42224-2) (Met 1-Val 712) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Fusion Tag: N-His-GST

Species: Human

Expressed Host: Baculovirus-Insect Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 92 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg of the protein as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 111 kDa

Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4

Reconstitution: Please refer to the printed manual for detailed information.

Background: STAT1 is a member of the STAT protein family. In response to cytokines and growth factors; STAT family members are phosphorylated by the receptor associated kinases; and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT1 can be activated by various ligands; including interferon-alpha; interferon-gamma; EGF; PDGF and IL6. It is a signal transducer and transcription activator that mediates cellular responses to interferons (IFNs); cytokine KITLG/SCF and other cytokines and growth factors. The phosphorylated STATs dimerize; associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor; that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes; which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma); STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF); migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes; inducing a cellular antiviral state. STAT1 becomes activated in response to KITLG/SCF and KIT signaling and may mediate cellular responses to activated FGFR1; FGFR2; FGFR3 and FGFR4. Defects in STAT1 can cause STAT1 deficiency complete and familial candidiasis type 7.

Research Area: N/A