Recombinant Human SPHK1/Sphingosine Kinase 1 Protein (His&GST Tag) | PKSH030316

Recombinant Human SPHK1/Sphingosine Kinase 1 Protein (His&GST Tag) | PKSH030316 | Gentaur US, UK & Europe Disrtribition

Synonyms: SPHK;SPHK1;SPK

Active Protein: N/A

Activity: A DNA sequence encoding the human SPHK1 (Q9NYA1-1)(Met1-Leu384) was expressed the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Protein Construction: A DNA sequence encoding the human SPHK1 (Q9NYA1-1)(Met1-Leu384) was expressed the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Fusion Tag: N-His & GST

Species: Human

Expressed Host: Baculovirus-Insect Cells

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Molecular Mass: 70.3 kDa

Formulation: Supplied as sterile 20mM Tris, 500mM NaCl, 3mM DTT, 10% glycerol, pH 8.0

Reconstitution: Not Applicable

Background: SPHK1; also known as sphingosine Kinase 1; catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P). S1P exhibits a broad spectrum of biological activities including cell proliferation; survival; migration; cytoskeletal organization; and morphogenesis. It is a ligand for cell surface G protein-coupled receptors. SPHK 1 is a potential therapeutic target for the control of cancer and inflammation. SPHK1 plays a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory; antiapoptotic; and immune processes.

Research Area: N/A