Recombinant Human Sonic Hedgehog/SHH Protein (aa 1-197, His Tag)(Active) | PKSH031645

Recombinant Human Sonic Hedgehog/SHH Protein (aa 1-197, His Tag)(Active) | PKSH031645 | Gentaur US, UK & Europe Disrtribition

Synonyms: Sonic Hedgehog Protein; SHH; HHG-1; SHH

Active Protein: Active protein

Activity: A DNA sequence encoding the amino acid sequence (Met 1-Gly 197) of human SHH (Q15465); that is Sonic hedgehog protein N-product; was fused with a polyhistidine tag at the C-terminus.

Protein Construction: A DNA sequence encoding the amino acid sequence (Met 1-Gly 197) of human SHH (Q15465); that is Sonic hedgehog protein N-product; was fused with a polyhistidine tag at the C-terminus.

Fusion Tag: C-His

Species: Human

Expressed Host: HEK293 Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 21 kDa

Formulation: Lyophilized from sterile PBS, pH 7.4

Reconstitution: Please refer to the printed manual for detailed information.

Background: Sonic HedgeHog; also known as sonic hedgehog protein; belongs to the hedgehog family. It cannot be detected in adult tissues while can be found in fetal intestine; liver; lung; and kidney. Sonic HedgeHog is a protein that is vital in guding the early embryo. It has been associated as the major inductive signal in patterning of the ventral neural tube; the anterior-posterior limb axis; and the ventral somites. Sonic HedgeHog intercellular signal is essential for a various patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites; and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Sonic HedgeHog binds to the patched receptor; which functions in association with smoothened; to activate the transcription of target genes. In the absence of sonic HedgeHog; patched receptor represses the constitutive signaling activity of smoothened. Sonic HedgeHog also regulates another factor; the gli oncogene. Defects in sonic hedgehog can cause microphthalmia isolated with coloboma type 5; triphalangeal thumb-polysyndactyly syndrome and holoprosencephaly type 3.

Research Area: Cancer, Developmental Biology, epigenetics and nuclear signal, metabolism, Stem cells