Recombinant Human SMYD3/ZMYND1 Protein (His&FLAG Tag) | PKSH031188

Recombinant Human SMYD3/ZMYND1 Protein (His&FLAG Tag) | PKSH031188 | Gentaur US, UK & Europe Disrtribition

Synonyms: bA74P14.1;KMT3E;ZMYND1;ZNFN3A1

Active Protein: N/A

Activity: A DNA sequence encoding the human SMYD3 isoform 1 (NP_001161212.1) (Met 1-Ser 428) was fused with the flag tag at the N-terminus. and the Histidine tag at the C-terminus.

Protein Construction: A DNA sequence encoding the human SMYD3 isoform 1 (NP_001161212.1) (Met 1-Ser 428) was fused with the flag tag at the N-terminus. and the Histidine tag at the C-terminus.

Fusion Tag: C-His & N-FLAG

Species: Human

Expressed Host: HEK293 Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 70 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 51.5 kDa

Formulation: Lyophilized from sterile 25mM Tris-HCl, 100mM NaCl, 20% glycerol, 3mM DTT, pH 8.0

Reconstitution: Please refer to the printed manual for detailed information.

Background: SET and MYND domain-containing protein 3, also known as Zinc finger MYND domain-containing protein 1, SMYD3, and ZMYND, is a member of the histone-lysine methyltransferase family. SMYD3 contains one MYND-type zinc finger and one SET domain. SMYD3 is a histone H3 lysine-4-specific methyltransferase. It is expressed in skeletal muscles and testis. It is overexpressed in a majority of colorectal carcinoma (CRC) and hepatocellular carcinoma (HCC). SMYD3 plays an important role in transcriptional regulation in human carcinogenesis. It activates the transcription of a set of downstream genes. Of these downstream genes, there are several oncogenes and genes associated with cell adhesion (including those of N-Myc, CrkL, Wnt10b, L-selectin, CD31 and galectin-4), which have been shown to have effects on cell viability, adhesion, migration and metastasis. Increased SMYD3 expression is essential for the proliferation of breast cancer cells. SMYD3 may be a promising new target of therapeutic intervention for the treatment of cancers or other pathological processes associated with cell adhesion and migration.

Research Area: Cancer, epigenetics and nuclear signal