Recombinant Human SMAD4 Protein (His Tag) | PKSH033066

Recombinant Human SMAD4 Protein (His Tag) | PKSH033066 | Gentaur US, UK & Europe Disrtribition

Synonyms: Mothers Against Decapentaplegic Homolog 4; MAD Homolog 4; Mothers Against DPP Homolog 4; Deletion Target in Pancreatic Carcinoma 4; SMAD Family Member 4; SMAD 4; Smad4; hSMAD4; SMAD4; DPC4; MADH4

Active Protein: N/A

Activity: Recombinant Human Mothers Against Decapentaplegic Homolog 4 is produced by our E.coli expression system and the target gene encoding Met1-Asp552 is expressed with a 6His tag at the C-terminus.

Protein Construction: Recombinant Human Mothers Against Decapentaplegic Homolog 4 is produced by our E.coli expression system and the target gene encoding Met1-Asp552 is expressed with a 6His tag at the C-terminus.

Fusion Tag: C-6His

Species: Human

Expressed Host: E.coli

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 85 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 61.5 kDa

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM TrisHCl，pH8.0.

Reconstitution: Please refer to the printed manual for detailed information.

Background: SMAD Family Member 4 (SMAD4) is a cytoplasmic protein that belongs to the Dwarfin/SMAD family. SMAD4 contains one MH1 (MAD homology 1) domain and one MH2 (MAD homology 2) domain. It is the component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. SMAD4 promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. SMAD4 may act as a tumor suppressor. It positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. Mutations or deletions in SMAD4 have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome.

Research Area: Signal Transduction, Cardiovascular, Cancer, epigenetics and nuclear signal, metabolism, Stem cells