Description
Recombinant Human SIGLEC2/CD22 Protein (Fc Tag) | PKSH033750 | Gentaur US, UK & Europe Disrtribition
Synonyms: B-cell receptor CD22; BL-CAM; B-lymphocyte cell adhesion molecule; CD22 antigenMGC130020; CD22 molecule; CD22; sialic acid binding Ig-like lectin 2; Siglec-2; SIGLEC2FLJ22814; T-cell surface antigen Leu-14;SIGLEC-2;Siglec-2
Active Protein: N/A
Activity: Recombinant Human CD22 is produced by our Mammalian expression system and the target gene encoding Asp20-Arg687 is expressed with a Fc tag at the C-terminus.
Protein Construction: Recombinant Human CD22 is produced by our Mammalian expression system and the target gene encoding Asp20-Arg687 is expressed with a Fc tag at the C-terminus.
Fusion Tag: C-Fc
Species: Human
Expressed Host: Human Cells
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Purity: > 95 % as determined by reducing SDS-PAGE.
Endotoxin: < 1.0 EU per μg as determined by the LAL method.
Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Molecular Mass: 102.3 kDa
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 .
Reconstitution: Please refer to the printed manual for detailed information.
Background: CD22 is a member of the immunoglobulin superfamily; SIGLEC family of lectins. It is first expressed in the cytoplasm of pro-B and pre-B cells; and on the surface as B cells mature to become IgD+. CD22 serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. In addition to its potential role as a mediator of intercellular interactions; signal transduction through CD22 can activate B cells and modulate antigen receptor signaling in vitro. The phenotype of CD22-deficient mice suggests that CD22 is primarily involved in the generation of mature B cells within the bone marrow; blood; and marginal zones of lymphoid tissues. CD22 recruits the tyrosine phosphatase Src homology 2 domain-containing phosphatase 1 (SHP-1) to immunoreceptor tyrosine-based inhibitory motifs (ITIMs) and inhibits B-cell receptor (BCR)-induced Ca2+ signaling on normal B cells. CD22 interacts specifically with ligands carrying alpha2-6-linked sialic acids. As an inhibitory coreceptor of the B-cell receptor (BCR); CD22 plays a critical role in establishing signalling thresholds for B-cell activation. Like other coreceptors; the ability of CD22 to modulate B-cell signalling is critically dependent upon its proximity to the BCR; and this in turn is governed by the binding of its extracellular domain to alpha2;6-linked sialic acid ligands. However; genetic studies in mice reveal that some CD22 functions are regulated by ligand binding; whereas other functions are ligand-independent and may only require expression of an intact CD22 cytoplasmic domain at the B-cell surface. CD19 regulates CD22 phosphorylation by augmenting Lyn kinase activity; while CD22 inhibits CD19 phosphorylation via SHP-1.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
Research Area: Cancer, immunology, Stem cells
