Recombinant Human SIAE Protein (Human Cells, His Tag) | PKSH033053

Recombinant Human SIAE Protein (Human Cells, His Tag) | PKSH033053 | Gentaur US, UK & Europe Disrtribition

Synonyms: Sialate O-Acetylesterase; H-Lse; Sialic Acid-Specific 9-O-Acetylesterase; SIAE; YSG2;AIS6;CSE-C;CSEC

Active Protein: N/A

Activity: Recombinant Human Sialate O-Acetylesterase is produced by our Mammalian expression system and the target gene encoding Ile24-Lys523 is expressed with a 6His tag at the C-terminus.

Protein Construction: Recombinant Human Sialate O-Acetylesterase is produced by our Mammalian expression system and the target gene encoding Ile24-Lys523 is expressed with a 6His tag at the C-terminus.

Fusion Tag: C-6His

Species: Human

Expressed Host: Human Cells

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Molecular Mass: 57.0 kDa

Formulation: Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 150mM NaCl, 10%Glycerol, pH7.5.

Reconstitution: Not Applicable

Background: Sialate O-Acetylesterase (SIAE) belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds. SIAE is widely expressed with high expression levels in the testis, prostate, and colon. SIAE catalyzes N-acetyl-O-acetylneuraminate and H2O to N-acetylneuraminate and acetate. SIAE removes O-acetyl ester groups from position 9 of the parent sialic acid, N-acetylneuraminic acid. SIAE down-regulates B lymphocyte antigen receptor signaling (involving CD22), and is required for immunological tolerance. Loss of function mutations in SIAE are much more frequently found in humans with autoimmune diseases especially rheumatoid arthritis and type 1 diabetes.

Research Area: Tags & Cell Markers