Recombinant Human SHMT1 Protein (His Tag) | PKSH033029

Recombinant Human SHMT1 Protein (His Tag) | PKSH033029 | Gentaur US, UK & Europe Disrtribition

Synonyms: Serine Hydroxymethyltransferase Cytosolic; SHMT; Glycine Hydroxymethyltransferase; Serine Methylase; SHMT1

Active Protein: N/A

Activity: Recombinant Human Serine Hydroxymethyltransferase Cytosolic is produced by our Mammalian expression system and the target gene encoding Met3-Phe483 is expressed with a 6His tag at the C-terminus.

Protein Construction: Recombinant Human Serine Hydroxymethyltransferase Cytosolic is produced by our Mammalian expression system and the target gene encoding Met3-Phe483 is expressed with a 6His tag at the C-terminus.

Fusion Tag: C-6His

Species: Human

Expressed Host: Human Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 53.9 kDa

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH7.4.

Reconstitution: Please refer to the printed manual for detailed information.

Background: Serine Hydroxymethyltransferase Cytosolic (SHMT1) is a member of the SHMT family. SHMT1 is a cytoplasmic protein and exists as a homotetramer. SHMT1 catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5, 10-methylene tetrahydrofolate. This reaction provides one carbon unit for the synthesis of methionine, thymidylate, and purines in the cytoplasm. A reduction in SHMT1 levels would result in less glycine that could affect the nervous system by acting as an agonist to the NMDA receptor and this could be a mechanism behind Smith-Magenis syndrome.

Research Area: Signal Transduction, metabolism,