Recombinant Human SERPINB9/PI-9 Protein (Baculovirus, His Tag) | PKSH030777

Recombinant Human SERPINB9/PI-9 Protein (Baculovirus, His Tag) | PKSH030777 | Gentaur US, UK & Europe Disrtribition

Synonyms: Cytoplasmic antiproteinase 3;Peptidase inhibitor 9;CAP3;PI-9;Serpin B9

Active Protein: N/A

Activity: A DNA sequence encoding the human SERPINB9 (P50453) (Glu 2-Pro 376) was expressed; with a polyhistidine tag at the N-terminus.

Protein Construction: A DNA sequence encoding the human SERPINB9 (P50453) (Glu 2-Pro 376) was expressed; with a polyhistidine tag at the N-terminus.

Fusion Tag: N-His

Species: Human

Expressed Host: Baculovirus-Insect Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 97 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg of the protein as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 44.5 kDa

Formulation: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 7.4

Reconstitution: Please refer to the printed manual for detailed information.

Background: SerpinB9; also known as Cytoplasmic antiproteinase 3; CAP-3; Peptidase inhibitor 9; SERPINB9 and PI-9; is a cytoplasm protein which belongs to the serpin family and Ov-serpin subfamily. Serpin-B9 ( CAP-3 / PI-9 ) is the only known human intracellular inhibitor of granzyme B (GrB); the effector molecule in immunity against cytomegalovirus (CMV) and in renal allograft rejection. Serpin-B9 and SPI-6 expression in immune-privileged cells; APCs; and CTLs protects these cells against the actions of granzyme B; and when expressed in tumor cells or virally infected hepatocytes; confers resistance to killing by CTL and NK cells. Expression of increasing levels of Serpin-B9 ( CAP-3 / PI-9 ) in target cells may progressively inhibit immune surveillance by blocking NK and CTL-induced cytotoxicity through the perforin / granzyme pathway and then through the Fas / FasL pathway. Serpin-B9 ( CAP-3 / PI-9 ) is selectively up-regulated in hepatocytes in response to infiltration of the liver by NK cells that express perforin and enzymatically active granzyme B. Upregulated expression of Serpin-B9 ( CAP-3 / PI-9 ) in NSCLC cells may serve to protect them from apoptosis induced by GrB.

Research Area: Cell biology, immunology