Recombinant Human S100B Protein (Fc Tag)(Active) | PKSH031789

Recombinant Human S100B Protein (Fc Tag)(Active) | PKSH031789 | Gentaur US, UK & Europe Disrtribition

Synonyms: Protein S100-B; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium-binding protein B; S100b; S100 beta; S100 calcium binding protein B;NEF;S100;S100-B;S100beta

Active Protein: Active protein

Activity: A DNA sequence encoding the human S100B (NP_006263.1) (Ser 2-Glu 92) was expressed with the fused Fc region of human IgG1 at the N-terminus.

Protein Construction: A DNA sequence encoding the human S100B (NP_006263.1) (Ser 2-Glu 92) was expressed with the fused Fc region of human IgG1 at the N-terminus.

Fusion Tag: N-Fc

Species: Human

Expressed Host: HEK293 Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 37.2 kDa

Formulation: Lyophilized from sterile PBS, pH 7.4

Reconstitution: Please refer to the printed manual for detailed information.

Background: S100B is a member of the S100 family of proteins containing two EF-hand-type calcium-binding motifs. S100B exerts both intracellular and extracellular functions. Intracellular S100B acts as a stimulator of cell proliferation and migration and an inhibitor of apoptosis and differentiation; which might have important implications during brain; cartilage and skeletal muscle development and repair; activation of astrocytes in the course of brain damage and neurodegenerative processes; and of cardiomyocyte remodeling after infarction; as well as in melanomagenesis and gliomagenesis. As an extracellular factor; S100B engages RAGE (receptor for advanced glycation end products) in a variety of cell types with different outcomes (i.e. beneficial or detrimental; pro-proliferative or pro-differentiative) depending on the concentration attained by the protein; the cell type and the microenvironment. This calcium binding astrocyte-specific cytokine; presents a marker of astrocytic activation and reflects CNS injury. The excellent sensitivity of S100B has enabled it to confirm the existence of subtle brain injury in patients with mild head trauma; strokes; and after successful resuscitation from cardiopulmonary arrest. Recent findings provide evidence; that S100B may decrease neuronal injury and/or contribute to repair following traumatic brain injury (TBI). Hence; S100B; far from being a negative determinant of outcome; as suggested previously in the human TBI and ischemia literature; is of potential therapeutic value that could improve outcome in patients who sustain various forms of acute brain damage.

Research Area: Cell biology, epigenetics and nuclear signal, Stem cells