Recombinant Human RPE Protein (His Tag) | PKSH030953

Recombinant Human RPE Protein (His Tag) | PKSH030953 | Gentaur US, UK & Europe Disrtribition

Synonyms: Ribulose-Phosphate 3-Epimerase; Ribulose-5-Phosphate-3-Epimerase; RPE; HUSSY-17;RPE2-1

Active Protein: N/A

Activity: A DNA sequence encoding the human RPE isoform 1 (NP_954699.1) (Ala 2-Arg 228) was expressed; with a polyhistidine tag at the N-terminus.

Protein Construction: A DNA sequence encoding the human RPE isoform 1 (NP_954699.1) (Ala 2-Arg 228) was expressed; with a polyhistidine tag at the N-terminus.

Fusion Tag: N-His

Species: Human

Expressed Host: HEK293 Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 94 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 27 kDa

Formulation: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0

Reconstitution: Please refer to the printed manual for detailed information.

Background: The "ribulose phosphate binding" superfamily defined by the Structural Classification of Proteins (SCOP) database is considered the result of divergent evolution from a common (beta/alpha)(8)-barrel ancestor. The superfamily includes d-ribulose 5-phosphate 3-epimerase (RPE); orotidine 5'-monophosphate decarboxylase (OMPDC); and 3-keto-l-gulonate 6-phosphate decarboxylase (KGPDC). Replication of the human genome requires the activation of thousands of replicons distributed along each one of the chromosomes. Each replicon contains an initiation; or origin; site; at which DNA synthesis begins. In enzymology; a L-ribulose-5-phosphate 3-epimerase is an enzyme that catalyzes the chemical reaction L-ribulose 5-phosphate to L-xylulose 5-phosphate. Hence; RPE has one substrate; L-ribulose 5-phosphate; and one product; L-xylulose 5-phosphate. RPE belongs to the family of isomerases; specifically those racemases and epimerases acting on carbohydrates and derivatives. The systematic name of this enzyme class is L-ribulose-5-phosphate 3-epimerase. Other names in common use include L-xylulose 5-phosphate 3-epimerase; UlaE; and SgaU.

Research Area: Signal Transduction, Cancer, metabolism,