Recombinant Human Ribonuclease 3/RNASE3 Protein (His Tag) | PKSH033002

Recombinant Human Ribonuclease 3/RNASE3 Protein (His Tag) | PKSH033002 | Gentaur US, UK & Europe Disrtribition

Synonyms: Eosinophil Cationic Protein; ECP; Ribonuclease 3; RNase 3; RNASE3; ECP; RNS3

Active Protein: N/A

Activity: Recombinant Human Ribonuclease 3 is produced by our Mammalian expression system and the target gene encoding Arg28-Ile160 is expressed with a 6His tag at the C-terminus.

Protein Construction: Recombinant Human Ribonuclease 3 is produced by our Mammalian expression system and the target gene encoding Arg28-Ile160 is expressed with a 6His tag at the C-terminus.

Fusion Tag: C-6His

Species: Human

Expressed Host: Human Cells

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Molecular Mass: 16.6 kDa

Formulation: Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 150mM NaCl, 1mMDTT, 10%Glycerol, pH7.5.

Reconstitution: Not Applicable

Background: Ribonuclease 3 (RNASE3) is a basic protein that is localized to the eosinophil primary matrix and belongs to the pancreatic ribonuclease family. RNASE3 is released during degranulation of eosinophils. RNASE3 possesses a wide variety of biological activities. RNASE3 interacts with bacterial lipopolysaccharide (LPS) and lipoteichoic acid (LTA). RNASE3 exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. It promotes E. coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content.

Research Area: N/A