Recombinant Human RHEB Protein (GST Tag) | PKSH033545

Recombinant Human RHEB Protein (GST Tag) | PKSH033545 | Gentaur US, UK & Europe Disrtribition

Synonyms: GTP-Binding Protein Rheb; Ras Homolog Enriched in Brain; RHEB; RHEB2

Active Protein: N/A

Activity: Recombinant Human RHEB is produced by our E.coli expression system and the target gene encoding Met1-Met184 is expressed with a GST tag at the N-terminus.

Protein Construction: Recombinant Human RHEB is produced by our E.coli expression system and the target gene encoding Met1-Met184 is expressed with a GST tag at the N-terminus.

Fusion Tag: N-GST

Species: Human

Expressed Host: E.coli

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per μg as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 20.4 kDa

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 10mM GSH, pH 8.0.

Reconstitution: Please refer to the printed manual for detailed information.

Background: GTP-Binding Protein Rheb (RHEB) is a member of the small GTPase superfamily and encodes a lipid-anchored; cell membrane protein with five repeats of the RAS-related GTP-binding region. Highest levels of RHEB can be found in the skeletal and cardiac muscle; and it is vital in the regulation of growth and cell cycle progression due to its role in the Insulin/TOR/S6K signaling pathway. RHEB stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling; and it activates the protein kinase activity of mTORC1. RHEB has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form; farnesylation of the protein is required for this activity.

Research Area: Signal Transduction, Cancer, metabolism,