Recombinant Human RET Kinase Protein (aa 658-1114, His & GST Tag)(Active) | PKSH030342

Recombinant Human RET Kinase Protein (aa 658-1114, His & GST Tag)(Active) | PKSH030342 | Gentaur US, UK & Europe Disrtribition

Synonyms: CDHF12;CDHR16;HSCR1;MEN2A;MEN2B;MTC1;PTC;RET-ELE1;RET51

Active Protein: Active protein

Activity: A DNA sequence encoding the cytoplasmic domain of human RET (P07949-1) (His 658-Ser 1114) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Protein Construction: A DNA sequence encoding the cytoplasmic domain of human RET (P07949-1) (His 658-Ser 1114) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Fusion Tag: N-His & GST

Species: Human

Expressed Host: Baculovirus-Insect Cells

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Molecular Mass: 76.7 kDa

Formulation: Supplied as sterile 20mM Tris, 500mM NaCl, 10% gly, pH 8.0

Reconstitution: Not Applicable

Background: RET proto-oncogene; also known as RET; is a cell-surface molecule that transduce signals for cell growth and differentiation. It contains 1 cadherin domain and 1 protein kinase domain. RET proto-oncogene belongs to the protein kinase superfamily; tyr protein kinase family. RET proto-oncogene is involved in numerous cellular mechanisms including cell proliferation; neuronal navigation; cell migration; and cell differentiation upon binding with glial cell derived neurotrophic factor family ligands. It phosphorylates PTK2/FAK1 and regulates both cell death/survival balance and positional information. RET is required for the molecular mechanisms orchestration during intestine organogenesis; involved in the development of enteric nervous system and renal organogenesis during embryonic life; promotes the formation of Peyer's patch-like structures; modulates cell adhesion via its cleavage; involved in the development of the neural crest. RET proto-oncogene is active in the absence of ligand; triggering apoptosis. RET acts as a dependence receptor; in the presence of the ligand GDNF in somatotrophs (within pituitary); promotes survival and down regulates growth hormone (GH) production; but triggers apoptosis in absence of GDNF. It also regulates nociceptor survival and size; triggers the differentiation of rapidly adapting (RA) mechanoreceptors; mediated several diseases such as neuroendocrine cancers. Defects in RET may cause colorectal cancer; hirschsprung disease type 1; medullary thyroid carcinoma; multiple neoplasia type 2B; susceptibility to pheochromocytoma; multiple neoplasia type 2A; thyroid papillary carcinoma and congenital central hypoventilation syndrome.Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Research Area: N/A