Recombinant Human PTGS2/COX2/PGHS-2 Protein (His Tag) | PKSH030929

Recombinant Human PTGS2/COX2/PGHS-2 Protein (His Tag) | PKSH030929 | Gentaur US, UK & Europe Disrtribition

Synonyms: COX-2;COX2;GRIPGHS;hCox-2;PGG/HS;PGHS-2;PHS-2

Active Protein: N/A

Activity: A DNA sequence encoding the human PTGS2 (NP_000954.1) (Met 1-Leu 604) was fused with a polyhistidine tag at the C-terminus.

Protein Construction: A DNA sequence encoding the human PTGS2 (NP_000954.1) (Met 1-Leu 604) was fused with a polyhistidine tag at the C-terminus.

Fusion Tag: C-His

Species: Human

Expressed Host: Baculovirus-Insect Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 68.5 kDa

Formulation: Lyophilized from sterile 50mM Tris, 100mM NaCl, 0.5mM PMSF, 10% glycerol, pH 8.0

Reconstitution: Please refer to the printed manual for detailed information.

Background: PTGS2, also known as COX-2, is s component of Prostaglandin-endoperoxide synthase (PTGS). PTGS, also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. PTGS2 is over expressed in many cancers. The overexpression of PTGS2 along with increased angiogenesis and GLUT-1 expression is significantly associated with gallbladder carcinomas. Furthermore the product of COX-2, PGH2 is converted by prostaglandin E2 synthase into PGE2, which in turn can stimulate cancer progression. Consequently inhibiting COX-2 may have benefit in the prevention and treatment of these types of cancer. PTGS2 is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. It mediates the formation of prostaglandins from arachidonate and may have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity.

Research Area: Signal Transduction, Cardiovascular, Cancer, metabolism,