Description
Recombinant Human PS6K/RPS6KB1 Protein (GST Tag) | PKSH031881 | Gentaur US, UK & Europe Disrtribition
Synonyms: p70 S6KA;p70(S6K)-alpha;p70-alpha;p70-S6K;PS6K;RPS6KB1;S6K;S6K-beta-1;S6K1;STK14A
Active Protein: N/A
Activity: A DNA sequence encoding the human RPS6KB1 (P23443-Alpha I) (Met1-Leu525) was fused with the GST tag at the N-terminus.
Protein Construction: A DNA sequence encoding the human RPS6KB1 (P23443-Alpha I) (Met1-Leu525) was fused with the GST tag at the N-terminus.
Fusion Tag: N-GST
Species: Human
Expressed Host: Baculovirus-Insect Cells
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Purity: > 90 % as determined by reducing SDS-PAGE.
Endotoxin: < 1.0 EU per µg as determined by the LAL method.
Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Molecular Mass: 85.4 kDa
Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly, 1mM GSH
Reconstitution: Please refer to the printed manual for detailed information.
Background: PS6K, also known as RPS6KB1, is a serine/threonine-protein kinase. It belongs to the RSK (ribosomal s6 kinase) family. Members of this family function in signal transduction. PS6K is an isoform of p70 ribosomal S6 kinase (S6K). S6K can be activated by mitogenic stimuli such as growth factors, insulin and cytokines. It phosphorylates the ribosomal protein S6. PS6K also phosphorylates other proteins such as elF4B, eEF2K and SKAR. It is a crucial effector of mTOR(rapamycin) signaling. PS6K is dissociated from the EIF3 complex and activated upon mitogenic stimulation, phosphorylation by the mammalian target of mTOR complex 1 (mTORC1). Its active form then phosphorylates and activates several substrates in the preinitiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. PS6K also functions in cell proliferation, cell growth and cell cycle progression.
Research Area: Signal Transduction, Cell biology, epigenetics and nuclear signal, metabolism,
