Recombinant Human PRMT5/SKB1 Protein (His & FLAG Tag) | PKSH031276

Recombinant Human PRMT5/SKB1 Protein (His & FLAG Tag) | PKSH031276 | Gentaur US, UK & Europe Disrtribition

Synonyms: HRMT1L5;IBP72;JBP1;SKB1;SKB1Hs

Active Protein: N/A

Activity: A DNA sequence encoding the human PRMT5 (NP_006100.2) (Ala 2-Leu 637) was expressed; fused with a polyhistidine tag at the C-terminus and a Flag tag at the N-terminus.

Protein Construction: A DNA sequence encoding the human PRMT5 (NP_006100.2) (Ala 2-Leu 637) was expressed; fused with a polyhistidine tag at the C-terminus and a Flag tag at the N-terminus.

Fusion Tag: C-His & N-FLAG

Species: Human

Expressed Host: HEK293 Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 85 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 75 kDa

Formulation: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 0.5mM EDTA, 0.5PMSF, 0.5mM, TCEP, 25% glycerol

Reconstitution: Please refer to the printed manual for detailed information.

Background: Methylation of arginine residues is a widespread post-translational modification of proteins catalyzed by a small family of PRMTs. The modification appears to regulate protein functions and interactions that affect gene regulation; signalling and subcellular localization of proteins and nucleic acids. Protein arginine methyltransferase 5 (PRMT5) is a member of the protein arginine N-methyltransferases (PRMT)family; and exists as at least homodimers and homotetramers; or homooligomers mediated by disulfide bonds and non-covalent association ubiquitously. PRMT5 specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); and thus plays a role in the assembly and biogenesis of snRNP core particles. PRMT5 methylates histone H2A and H4 'Arg-3' during germ cell development; as well as histone H3 'Arg-8'; which may repress transcription. PRMT5 also methylates SUPT5H and regulates its transcriptional elongation properties. Additionally; it is also suggested that PRMT5 negatively regulates cyclin E1 promoter activity and cellular proliferation.

Research Area: epigenetics and nuclear signal