Description
Recombinant Human PRDM2/RIZ1 Protein (GST Tag) | PKSH031204 | Gentaur US, UK & Europe Disrtribition
Synonyms: HUMHOXY1;KMT8;MTB-ZF;RIZ;RIZ1;RIZ2
Active Protein: N/A
Activity: A DNA sequence encoding the N-terminal segment of human PRDM2 (NP_036363.2) (Met 1-Ala 200); containing the SET domian; was fused with the GST tag at the N-terminus.
Protein Construction: A DNA sequence encoding the N-terminal segment of human PRDM2 (NP_036363.2) (Met 1-Ala 200); containing the SET domian; was fused with the GST tag at the N-terminus.
Fusion Tag: N-GST
Species: Human
Expressed Host: E.coli
Shipping: This product is provided as lyophilized powder which is shipped with ice packs.
Purity: > 86 % as determined by reducing SDS-PAGE.
Endotoxin: Please contact us for more information.
Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Molecular Mass: 49.6 kDa
Formulation: Lyophilized from sterile 20mM Tris, 150mM NaCl, 0.5mM DTT, 0.5mM GSH, pH 8.0
Reconstitution: Please refer to the printed manual for detailed information.
Background: PR domain containing 2; with ZNF domain (PRDM2); also known as zinc finger protein RIZ; is a member of histone methyltransferase (HMT) class enzymes that methylate lysine residues of histones or proteins. HMTs contain a conserved catalytic core termed the SET domain; which shares sequence homology with an independently described sequence motif; the PR domain. PRDM2 contains 8 C2H2-type zinc fingers and a distinct SET domain; and is highly expressed in retinoblastoma cell lines and in brain tumors; as well as in a number of other cell lines and in brain; heart; skeletal muscle; liver and spleen. PRDM2 is a S-adenosyl-L-methionine-dependent histone methyltransferase that specifically methylates 'Lys-9' of histone H3; and is identified as a tumor suppressor. It is reported that intact PR( SET) sequence is required for tumor suppression functions; mutations in the PR domain caused activity reduction in human cancers. Also; S-adenosylhomocysteine or methyl donor deficiency inhibits RIZ1 and other H3 lysine 9 methylation activities. PRDM2 may also function as a DNA-binding transcription factor. It Binds to the macrophage-specific TPA-responsive element (MTE) of the HMOX1 (heme oxygenase 1) gene and act as a transcriptional activator. In addition; PRDM2 (RIZ) is able to binds to the retinoblastoma protein (RB) and also Interacts with GATA3.
Research Area: Neuroscience, Cancer, epigenetics and nuclear signal
