Recombinant Human PRC1 Protein (His Tag) | PKSH030666

Recombinant Human PRC1 Protein (His Tag) | PKSH030666 | Gentaur US, UK & Europe Disrtribition

Synonyms: ASE1

Active Protein: N/A

Activity: A DNA sequence encoding the human PRC1 isoform 1 (NP_003972.1) (Met 1-Ser 620) was expressed, with a polyhistidine tag at the N-terminus.

Protein Construction: A DNA sequence encoding the human PRC1 isoform 1 (NP_003972.1) (Met 1-Ser 620) was expressed, with a polyhistidine tag at the N-terminus.

Fusion Tag: N-His

Species: Human

Expressed Host: Baculovirus-Insect Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 74 kDa

Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 20% gly, 3mM DTT

Reconstitution: Please refer to the printed manual for detailed information.

Background: PRC1 (protein regulator of cytokinesis 1) is a key regulator of cytokinesis that cross-links antiparrallel microtubules at an average distance of 35 nM. It is essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. PRC1 is required for KIF14 localization to the central spindle and midbody. It is also required to recruit PLK1 to the spindle. PRC1 stimulates PLK1 phosphorylation of RACGAP1 to allow recruitment of ECT2 to the central spindle. It is a homodimer and interacts with the C-terminal Rho-GAP domain and the basic region of RACGAP1. The interaction with RACGAP1 inhibits its GAP activity towards CDC42 in vitro, which may be required for maintaining normal spindle morphology. PRC1 also interacts separately via its N-terminal region with the C-terminus of CENPE, KIF4A and KIF23 during late mitosis. It interacts with KIF14, IF20A and PLK1.

Research Area: Cell biology