Recombinant Human PPP3CA/CALNA Protein (His Tag)(Active) | PKSH030577

Recombinant Human PPP3CA/CALNA Protein (His Tag)(Active) | PKSH030577 | Gentaur US, UK & Europe Disrtribition

Synonyms: CALN;CALNA;CALNA1;CCN1;CNA1;PPP2B

Active Protein: Active protein

Activity: A DNA sequence encoding the human PPP3CA (Q08209-1) (Ser2-Gln521) was fused with a polyhistide tag at the N-terminus.

Protein Construction: A DNA sequence encoding the human PPP3CA (Q08209-1) (Ser2-Gln521) was fused with a polyhistide tag at the N-terminus.

Fusion Tag: N-His

Species: Human

Expressed Host: Baculovirus-Insect Cells

Shipping: This product is provided as lyophilized powder which is shipped with ice packs.

Purity: > 94 % as determined by reducing SDS-PAGE.

Endotoxin: < 1.0 EU per µg as determined by the LAL method.

Stability and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Molecular Mass: 60.8 kDa

Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% glycerol

Reconstitution: Please refer to the printed manual for detailed information.

Background: PPP3CA, also known as protein phosphatase 2B, is a member of the PPP phosphatase family, PP-2B subfamily. It is the alpha catalytic subunit of protein phosphatase 2B (PP2B). PP2B is a holoenzyme that is comprised of a catalytic subunit associated with regulatory subunits. It is a calcium regulated enzyme that is activated by calmodulin and participates in the signaling cascades involved in development of the nervous, cardiovascular, and musculoskeletal systems. PPP3CA activates the T cells of the immune system and can be blocked by drugs. It also activates NFATc (a transcription factor) by dephosphorylating it. The activated NFATc is subsequently translocated into the nucleus, where it upregulates the expression of interleukin 2. PPP3CA interacts with CRTC2, MYOZ1, MYOZ2 and MYOZ3. It also interacts with DNM1L. The interaction dephosphorylates DNM1L and regulates its translocation to mitochondria.

Research Area: N/A